Skip to main content
Addgene

pWH1266-Apra-sgRNA
(Plasmid #218372)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218372 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pWH1266
  • Backbone size w/o insert (bp) 9200
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Other growth strain: Acinetobacter
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA expression cassette
  • gRNA/shRNA sequence
    Unspecified
  • Species
    Synthetic
  • Insert Size (bp)
    20
  • Promoter J23119

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gebhardt lab ID # pMJG440. 5' Cloning Site: SpeI (Not Destroyed), 3' Cloning Site: ApaI (Not Destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWH1266-Apra-sgRNA was a gift from Michael Gebhardt (Addgene plasmid # 218372 ; http://n2t.net/addgene:218372 ; RRID:Addgene_218372)

  • For your References section:

    A series of vectors for inducible gene expression in multidrug-resistant Acinetobacter baumannii. Intorcia V, Sava RL, Schroeder GP, Gebhardt MJ. Appl Environ Microbiol. 2024 Sep 18;90(9):e0047424. doi: 10.1128/aem.00474-24. Epub 2024 Aug 20. 10.1128/aem.00474-24 PubMed 39162403
Related items: