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Addgene

pUC18T-mini-Tn7T-hph-Ptac-VSVG
(Plasmid #218365)

Ordering

This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 218365 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC18T-mini-Tn7T
  • Vector type
    Bacterial Expression
  • Selectable markers
    Hygromycin
  • Tag / Fusion Protein
    • VSVG (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • VSVG (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gebhardt lab ID # pMJG520.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC18T-mini-Tn7T-hph-Ptac-VSVG was a gift from Michael Gebhardt (Addgene plasmid # 218365 ; http://n2t.net/addgene:218365 ; RRID:Addgene_218365)
  • For your References section:

    A series of vectors for inducible gene expression in multidrug-resistant Acinetobacter baumannii. Intorcia V, Sava RL, Schroeder GP, Gebhardt MJ. Appl Environ Microbiol. 2024 Sep 18;90(9):e0047424. doi: 10.1128/aem.00474-24. Epub 2024 Aug 20. 10.1128/aem.00474-24 PubMed 39162403