pBABE
(Plasmid #21836)

• Depositing Lab: David Ron
• Publication: David Ron plasmids (unpublished)

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 4569
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: EGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP
• Insert Size (bp): 874
• Tag / Fusion Protein:
  • farnysylation signal (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (destroyed during cloning)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: SV40pro-F

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Puro resistance gene of pBABE puro was removed by Hind3-Cla1 digestion and replaced by the CDS of a EGFP that has a farnysylation signal in its C-term. The membrane association of EGFP is instrumental in detecting the protein in FACScan procedures that would otherwise lead to loss of GFP fluorescence.