MyD116.delC.pBABEpu
(Plasmid #21835)

• Depositing Lab: David Ron
• Publication: Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22.

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GADD34
• Alt name: MyD116
• Species: M. musculus (mouse)
• Insert Size (bp): 1914
• Entrez Gene: Ppp1r15a (a.k.a. 9630030H21, Gadd34, Myd116)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnbI (destroyed during cloning)
• 3′ cloning site: SnbI (destroyed during cloning)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The construct was created by recovering the Sma1 fragment of mMyD116 and ligating it into the unique SnB1 site of pBABEpu. The stop codo is deep in the downstream SV40 promoter sequence andowing the encoded poly-peptide with a tail of nonesense sequence, but it does serve as an expressed negative control for the effects of GADD34.