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Addgene

pLentiCRISPRRv2_sgTom
(Plasmid #218346)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218346 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLentiCRISPRv2
  • Backbone size w/o insert (bp) 14873
  • Total vector size (bp) 13009
  • Modifications to backbone
    Inserted the tdTomato targeted sgRNA sequence: GCCACGAGTTCGAGAT using BsmB1
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Inserted sgRNA targetting tdTomato
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    25
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmB1 (not destroyed)
  • 3′ cloning site BsmB1 (not destroyed)
  • 5′ sequencing primer gaCTATCATATGCTTACCGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiCRISPRRv2_sgTom was a gift from Seth Parker (Addgene plasmid # 218346 ; http://n2t.net/addgene:218346 ; RRID:Addgene_218346)

  • For your References section:

    Restricting lysine normalizes toxic catabolites associated with ALDH7A1 deficiency in cells and mice. Johal AS, Al-Shekaili HH, Abedrabbo M, Kehinde AZ, Towriss M, Koe JC, Hewton KG, Thomson SB, Ciernia AV, Leavitt B, Parker SJ. Cell Reports, Volume 43, Issue 12, 115069 10.1016/j.celrep.2024.115069
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