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Addgene

MyD116.PFLAG.CMV2
(Plasmid #21834)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21834 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV2
  • Backbone size w/o insert (bp) 4724
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GADD34
  • Alt name
    MyD116
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2144
  • Entrez Gene
    Ppp1r15a (a.k.a. 9630030H21, Gadd34, Myd116)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-PA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MyD116.PFLAG.CMV2 was a gift from David Ron (Addgene plasmid # 21834 ; http://n2t.net/addgene:21834 ; RRID:Addgene_21834)

  • For your References section:

    Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2alpha. Novoa I, Zeng H, Harding HP, Ron D. J Cell Biol. 2001 May 28. 153(5):1011-22. 10.1083/jcb.153.5.1011 PubMed 11381086
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