pICOZ-MAG[EF1α-CD19 CD28Z]
(Plasmid
#218333)
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PurposeDonor plasmid of MAG Transposon with CD19 CAR mammalian expression cassette
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218333 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepICOZ
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCD19 CAR mammalian expression cassette
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pICOZ-MAG[EF1α-CD19 CD28Z] was a gift from Haoyi Wang (Addgene plasmid # 218333 ; http://n2t.net/addgene:218333 ; RRID:Addgene_218333) -
For your References section:
Heterologous survey of 130 DNA transposons in human cells highlights their functional divergence and expands the genome engineering toolbox. Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao Y, Chen Q, Guo B, Wang H, Zhang YE. Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 10.1016/j.cell.2024.05.007. Epub 2024 Jun 5. 10.1016/j.cell.2024.05.007 PubMed 38843831