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Addgene

TLS 2: hTLS.pCDNA1
(Plasmid #21828)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21828 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA1
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    This plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TLS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Entrez Gene
    FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human TLS full-length cDNA in pCDNA1 expression vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TLS 2: hTLS.pCDNA1 was a gift from David Ron (Addgene plasmid # 21828 ; http://n2t.net/addgene:21828 ; RRID:Addgene_21828)

  • For your References section:

    Fusion of CHOP to a novel RNA-binding protein in human myxoid liposarcoma. Crozat A, Aman P, Mandahl N, Ron D. Nature. 1993 Jun 17;363(6430):640-4. 10.1038/363640a0 PubMed 8510758
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