SV40 1: pBSSVD2005
(Plasmid #21826)

• Depositing Lab: David Ron
• Publication: SV40 large T antigen for immortalizing cells (unpublished)

Backbone

• Vector backbone: Bluescribe
• Backbone size w/o insert (bp): 3200
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SV40 Large T
• Alt name: Simian virus-40 large-T antigen
• Species: simian virus
• Insert Size (bp): 2800
• Mutation: likely expresses aa109-708 of the large T antigen
• Entrez Gene: SV40gp6 (a.k.a. SV40gp6)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • Protocol for Immortalizing MEFs
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 31 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SV40 large T antigen for immortalizing cells. See Author's Map for more information.

Please note that this plasmid likely expreses a truncated SV40 large T antigen protein starting with the methionine at amino acid 109, when compared to GenBank reference sequence YP_003708382.1 (see NC_001669.1 for the corresponding nucleotide sequence). The plasmid functions as described to immortalize MEFs.