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SREC 2: abu-1::abu-1::gfp
(Plasmid #21825)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21825 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD95_75
  • Backbone manufacturer
    Available at Addgene (#1494)
  • Backbone size w/o insert (bp) 4487
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    abu-1 promoter and coding region
  • Alt name
    AC3.3
  • Alt name
    abu1
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2700
  • Entrez Gene
    abu-1
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pPD95.75 was a gift from Dr. Andy Fire

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing results show there is a S408P substitution in amino acid sequence in the coding region.

abu-1::abu-1::gfp C. elegans reporter gene. The promoter and coding region of abu-1 was ligated in frame with GFP to produce abu1::abu-1::gfp

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SREC 2: abu-1::abu-1::gfp was a gift from David Ron (Addgene plasmid # 21825 ; http://n2t.net/addgene:21825 ; RRID:Addgene_21825)

  • For your References section:

    A survival pathway for Caenorhabditis elegans with a blocked unfolded protein response. Urano F, Calfon M, Yoneda T, Yun C, Kiraly M, Clark SG, Ron D. J Cell Biol. 2002 Aug 19. 158(4):639-46. 10.1083/jcb.200203086 PubMed 12186849
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