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Addgene

pCW57-CMV-MAAP-SL08
(Plasmid #218247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW57.1
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Membrane-Associated Accessory Protein Variant SL08 driven by CMV promoter
  • Alt name
    MAAP-SL08
  • Alt name
    MAAP-SL08V2
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57-CMV-MAAP-SL08 was a gift from David Schaffer (Addgene plasmid # 218247 ; http://n2t.net/addgene:218247 ; RRID:Addgene_218247)

  • For your References section:

    Evolving membrane-associated accessory protein variants for improved adeno-associated virus production. Schieferecke AJ, Lee H, Chen A, Kilaru V, Krish Williams J, Schaffer DV. Mol Ther. 2024 Feb 7;32(2):340-351. doi: 10.1016/j.ymthe.2023.12.015. Epub 2023 Dec 18. 10.1016/j.ymthe.2023.12.015 PubMed 38115579
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