pCW57-CMV-MAAP2
(Plasmid
#218244)
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Purpose1) Ectopic expression of MAAP2 protein in mammalian cells. 2) Packaging MAAP2 sequence into lentivirus for stable expression in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218244 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCW57.1
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMembrane-Associated Accessory Protein from Adeno-Associated Virus 2 driven by CMV promoter
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Alt nameMAAP2
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Alt nameMAAP-WT2
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SpeciesAdeno-Associated Virus
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCW57-CMV-MAAP2 was a gift from David Schaffer (Addgene plasmid # 218244 ; http://n2t.net/addgene:218244 ; RRID:Addgene_218244) -
For your References section:
Evolving membrane-associated accessory protein variants for improved adeno-associated virus production. Schieferecke AJ, Lee H, Chen A, Kilaru V, Krish Williams J, Schaffer DV. Mol Ther. 2024 Feb 7;32(2):340-351. doi: 10.1016/j.ymthe.2023.12.015. Epub 2023 Dec 18. 10.1016/j.ymthe.2023.12.015 PubMed 38115579