SREC 1: abu-1::gfp
(Plasmid #21824)

• Depositing Lab: David Ron
• Publication: Urano et al J Cell Biol. 2002 Aug 19. 158(4):639-46.

Backbone

• Vector backbone: pPD95_75
• Backbone manufacturer: Available at Addgene (#1494)
• Backbone size w/o insert (bp): 4487
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: abu-1 promoter
• Alt name: AC3.3
• Alt name: abu1
• Species: C. elegans (nematode)
• Insert Size (bp): 1300
• Entrez Gene: abu-1
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13r

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pPD95.75 was a gift from Dr. Andy Fire

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results show several mismatches with author's sequence.

abu-1::gfp C. elegans reporter gene. A 1.3-kb fragment of C. elegans genomic DNA immediately 5' of the predicted initiation ATG codon of abu-1 (AC3.3) was amplified by PCR using the oligonucleotides AC3.1S (5'-GGCATTGTGGCACGCATTGAACTG-3') and AC3Bam.2AS (5'-GATAGGATCCATTGTTAATATGCTTGAAGAGCTGC-3') and ligated in frame with the GFP coding region in the plasmid of pPD95.75.