piggyFlex
(Plasmid
#218234)
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Purpose(Empty Backbone) A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218234 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePB-U6-puroEGFP-v4
- Backbone size (bp) 7976
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Vector typeCRISPR
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Selectable markersPuromycin ; EGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector must be co-transfected with another vector containing the piggybac transposase in order to integrate into the genome.
Please visit https://doi.org/10.1101/2023.03.28.534017 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
piggyFlex was a gift from Jay Shendure (Addgene plasmid # 218234 ; http://n2t.net/addgene:218234 ; RRID:Addgene_218234) -
For your References section:
Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements. Chardon FM, McDiarmid TA, Page NF, Martin B, Domcke S, Regalado SG, Lalanne JAE, Calderon D, Starita LM, Sanders SJ, Ahituv N, Shendure J. bioRxiv [Preprint]. 2023 Mar 28:2023.03.28.534017. doi: 10.1101/2023.03.28.534017. 10.1101/2023.03.28.534017 PubMed 37034704
Map uploaded by the depositor.
