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Addgene

piggyFlex
(Plasmid #218234)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218234 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-U6-puroEGFP-v4
  • Backbone size (bp) 7976
  • Vector type
    CRISPR
  • Selectable markers
    Puromycin ; EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector must be co-transfected with another vector containing the piggybac transposase in order to integrate into the genome.

Please visit https://doi.org/10.1101/2023.03.28.534017 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piggyFlex was a gift from Jay Shendure (Addgene plasmid # 218234 ; http://n2t.net/addgene:218234 ; RRID:Addgene_218234)

  • For your References section:

    Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements. Chardon FM, McDiarmid TA, Page NF, Martin B, Domcke S, Regalado SG, Lalanne JAE, Calderon D, Starita LM, Sanders SJ, Ahituv N, Shendure J. bioRxiv [Preprint]. 2023 Mar 28:2023.03.28.534017. doi: 10.1101/2023.03.28.534017. 10.1101/2023.03.28.534017 PubMed 37034704
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