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Addgene

8TBX
(Plasmid #218228)

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Item Catalog # Description Quantity Price (USD)
Plasmid 218228 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFBOH-MHL
  • Vector type
    Baculovirus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDX1
  • Alt name
    PDB: 8TBX
  • Alt name
    DDX1:PBC037-A11:C247374
  • Species
    H. sapiens (human)
  • Entrez Gene
    DDX1 (a.k.a. DBP-RB, UKVH5d)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8TBX was a gift from Cheryl Arrowsmith (Addgene plasmid # 218228 ; http://n2t.net/addgene:218228 ; RRID:Addgene_218228)
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