Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
(Plasmid
#218205)
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PurposeZebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218205 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneZED_vector
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Backbone manufacturerBessa et al, 2009 (PMID 19653328)
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Vector typeZebrafish expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Gene/Insert
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Gene/Insert name-2.1prox1a
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SpeciesD. rerio (zebrafish)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.08.21.550483 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2 was a gift from Katarzyna Koltowska (Addgene plasmid # 218205 ; http://n2t.net/addgene:218205 ; RRID:Addgene_218205) -
For your References section:
Multiple cis-regulatory elements control prox1a expression in distinct lymphatic vascular beds. Panara V, Yu H, Peng D, Staxang K, Hodik M, Filipek-Gorniok B, Kazenwadel J, Skoczylas R, Mason E, Allalou A, Harvey NL, Haitina T, Hogan BM, Koltowska K. Development. 2024 May 1;151(9):dev202525. doi: 10.1242/dev.202525. Epub 2024 May 9. 10.1242/dev.202525 PubMed 38722096
