pET-29b(+)_His-CBD-SpEndoH
(Plasmid
#218196)
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PurposeBacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218196 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-29b(+)
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsFor expression, grow in your preferred BL21(DE3) or related strain. Induce with 0.5 mM IPTG at 18C overnight or in autoinduction media at 18C overnight.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEndoH
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Alt nameD_pET-29b(+)_His-CBD-SpEndoH
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SpeciesStreptomyces plicatus
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Mutationcodon optimized Ecoli, residues 45-313
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GenBank IDAAA26738.1 P04067
- Promoter T7
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Tag
/ Fusion Protein
- tufA-His-CBD (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer T7promoter
- 3′ sequencing primer T7terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-29b(+)_His-CBD-SpEndoH was a gift from Florence Pojer (Addgene plasmid # 218196 ; http://n2t.net/addgene:218196 ; RRID:Addgene_218196)
Map uploaded by the depositor.
