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pET-29b(+)_His-CBD-EmEndoF1
(Plasmid #218195)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218195 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-29b(+)
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For expression, grow in your preferred BL21(DE3) or related strain. Induce with 0.5 mM IPTG at 18C overnight or in autoinduction media at 18C overnight.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EndoF1
  • Alt name
    C_pET-29b(+)_His-CBD-EmEndoF1
  • Species
    Elizabethkingia miricola
  • Mutation
    codon optimized Ecoli, residues 51-339
  • GenBank ID
    AAA24922.1 P36911
  • Promoter T7
  • Tag / Fusion Protein
    • tufA-His-CBD (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-29b(+)_His-CBD-EmEndoF1 was a gift from Florence Pojer (Addgene plasmid # 218195 ; http://n2t.net/addgene:218195 ; RRID:Addgene_218195)
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