pET-29b(+)_His-CBD-3C-EmPNGaseF
(Plasmid #218193)

• Purpose: Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF
• Depositing Lab: Florence Pojer
• Publication: Deglycosylation Toolkit (unpublished)

Backbone

• Vector backbone: pET-29b(+)
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For expression, grow in your preferred BL21(DE3) or related strain. Induce with 0.5 mM IPTG at 18C overnight or in autoinduction media at 18C overnight.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PNGaseF
• Alt name: A_pET-29b(+)_His-CBD-3C-EmPNGaseF
• Species: Elizabethkingia miricola
• Mutation: codon optimized Ecoli, residues 41-354
• GenBank ID: P21163 AAA85323
• Promoter: T7
• Tag / Fusion Protein:
  • tufA-His-CBD-3C (N terminal on insert)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: T7promoter
• 3′ sequencing primer: T7terminator

Resource Information

• Supplemental Documents:
  • A_pET-29b(+)_His-CBD-3C-EmPNGaseF.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry