MBP-Benzonase
(Plasmid
#218192)
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PurposeBacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218192 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMAL2-PE
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Backbone manufacturerNEB
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)T7 Express Iq lysY
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namenucA
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SpeciesSerratia marcescens
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GenBank IDAAA26560.1 P13717
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Tag
/ Fusion Protein
- MBP (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid expresses a nuclease. Leaky expression of the nuclease may degrade extracted plasmid DNA over time. It is recommended that this plasmid be maintained in the provided host strain. If plasmid is extracted, use it immediately.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MBP-Benzonase was a gift from Cheryl Arrowsmith (Addgene plasmid # 218192 ; http://n2t.net/addgene:218192 ; RRID:Addgene_218192)