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Addgene

MBP-Benzonase
(Plasmid #218192)

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Item Catalog # Description Quantity Price (USD)
Plasmid 218192 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pMAL2-PE
  • Backbone manufacturer
    NEB
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    T7 Express Iq lysY
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    nucA
  • Species
    Serratia marcescens
  • GenBank ID
    AAA26560.1 P13717
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid expresses a nuclease. Leaky expression of the nuclease may degrade extracted plasmid DNA over time. It is recommended that this plasmid be maintained in the provided host strain. If plasmid is extracted, use it immediately.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MBP-Benzonase was a gift from Cheryl Arrowsmith (Addgene plasmid # 218192 ; http://n2t.net/addgene:218192 ; RRID:Addgene_218192)
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