GEARBOCS-SPARCL1-N-HATag
(Plasmid
#218185)
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PurposeTo tag Hevin with HA at the N-terminal
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneGEARBOCS 2.0
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Vector typeAAV, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRNA
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gRNA/shRNA sequenceCGCTACAGTCCCCATTGCCG
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SpeciesM. musculus (mouse)
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Entrez GeneSparcl1 (a.k.a. Ecm2, Sc1, hevin, mast9)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.01.17.524433 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GEARBOCS-SPARCL1-N-HATag was a gift from Cagla Eroglu (Addgene plasmid # 218185 ; http://n2t.net/addgene:218185 ; RRID:Addgene_218185) -
For your References section:
GEARBOCS: An Adeno Associated Virus Tool for In Vivo Gene Editing in Astrocytes. Bindu DS, Tan CX, Savage JT, Eroglu C. bioRxiv. 2023 Jan 19:2023.01.17.524433. doi: 10.1101/2023.01.17.524433. Preprint. 10.1101/2023.01.17.524433 PubMed 36711516