STREP-SUMO-FLAG-SeKaiC
(Plasmid #218179)

• Purpose: WT SeKaiC for phosphorylation/dephosphorylation study
• Depositing Lab: Andy LiWang
• Publication: Kim et al Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12825-30. doi: 10.1073/pnas.0800526105. Epub 2008 Aug 26.

Backbone

• Vector backbone: pET28b(+)
• Total vector size (bp): 7218
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: STREP-SUMO-FLAG-SeKaiC
• Alt name: WT-SeKaiC
• Species: Synechococcus elongatus
• Insert Size (bp): 7218
• Tag / Fusion Protein:
  • STREP-Tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: NdeI (unknown if destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mingxu Fang

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry