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STREP-SUMO-FLAG-SeKaiC
(Plasmid #218179)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218179 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b(+)
  • Total vector size (bp) 7218
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STREP-SUMO-FLAG-SeKaiC
  • Alt name
    WT-SeKaiC
  • Species
    Synechococcus elongatus
  • Insert Size (bp)
    7218
  • Tag / Fusion Protein
    • STREP-Tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site NdeI (unknown if destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mingxu Fang

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    STREP-SUMO-FLAG-SeKaiC was a gift from Andy LiWang (Addgene plasmid # 218179 ; http://n2t.net/addgene:218179 ; RRID:Addgene_218179)

  • For your References section:

    The day/night switch in KaiC, a central oscillator component of the circadian clock of cyanobacteria. Kim YI, Dong G, Carruthers CW Jr, Golden SS, LiWang A. Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12825-30. doi: 10.1073/pnas.0800526105. Epub 2008 Aug 26. 10.1073/pnas.0800526105 PubMed 18728181
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