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Addgene

pLenti-Puro 2xStrep-HPC4-Gb5
(Plasmid #218177)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218177 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti puro
  • Total vector size (bp) 8305
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G beta subunit 5
  • Species
    H. sapiens (human)
  • Entrez Gene
    GNB5 (a.k.a. GB5, HG2E, IDDCA, LADCI, LDMLS1, LDMLS2, gbeta5)
  • Tags / Fusion Proteins
    • 2x Strep tag (N terminal on insert)
    • HPC4 (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-Puro 2xStrep-HPC4-Gb5 was a gift from Barry Willardson (Addgene plasmid # 218177 ; http://n2t.net/addgene:218177 ; RRID:Addgene_218177)

  • For your References section:

    Visualizing the chaperone-mediated folding trajectory of the G protein beta5 beta-propeller. Wang S, Sass MI, Kwon Y, Ludlam WG, Smith TM, Carter EJ, Gladden NE, Riggi M, Iwasa JH, Willardson BM, Shen PS. Mol Cell. 2023 Nov 2;83(21):3852-3868.e6. doi: 10.1016/j.molcel.2023.09.032. Epub 2023 Oct 17. 10.1016/j.molcel.2023.09.032 PubMed 37852256
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