pcDNA3.1 PhLP1-myc-6xHis
(Plasmid
#218176)
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PurposeExpresses phosducin-like protein 1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218176 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Total vector size (bp) 6354
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePhosducin like protein 1
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Alt namePhLP1
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Alt namePDCL
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SpeciesH. sapiens (human)
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Entrez GenePDCL (a.k.a. PhLP)
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Tags
/ Fusion Proteins
- Myc (C terminal on insert)
- 6x His (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 PhLP1-myc-6xHis was a gift from Barry Willardson (Addgene plasmid # 218176 ; http://n2t.net/addgene:218176 ; RRID:Addgene_218176) -
For your References section:
Visualizing the chaperone-mediated folding trajectory of the G protein beta5 beta-propeller. Wang S, Sass MI, Kwon Y, Ludlam WG, Smith TM, Carter EJ, Gladden NE, Riggi M, Iwasa JH, Willardson BM, Shen PS. Mol Cell. 2023 Nov 2;83(21):3852-3868.e6. doi: 10.1016/j.molcel.2023.09.032. Epub 2023 Oct 17. 10.1016/j.molcel.2023.09.032 PubMed 37852256