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Addgene

pYPQ121-Split-TALED
(Plasmid #218171)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218171 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ121
  • Vector type
    Plant Expression ; Plastid base editing

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site AatII (unknown if destroyed)
  • 5′ sequencing primer TGTAAAACGACGGCCAGT
  • 3′ sequencing primer CAGGAAACAGCTATGAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ121-Split-TALED was a gift from Yiping Qi (Addgene plasmid # 218171 ; http://n2t.net/addgene:218171 ; RRID:Addgene_218171)

  • For your References section:

    Broad range plastid genome editing with monomeric TALE-linked cytosine and dual base editors. Wang X, Fang T, Lu J, Tripathi L, Qi Y. Plant Biotechnol J. 2024 May 6. doi: 10.1111/pbi.14358. 10.1111/pbi.14358 PubMed 38709858
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