pOET5 CVB3-VLP
(Plasmid #218146)

• Purpose: Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).
• Depositing Lab: Minna Hankaniemi
• Publication: Plasmid-based production of noro, rota, and enterovirus-like particles in insect cells (unpublished)

Backbone

• Vector backbone: pOET5.1
• Backbone manufacturer: Oxford Expression Technologies
• Backbone size w/o insert (bp): 5203
• Total vector size (bp): 9709
• Modifications to backbone: P10 promoter replaced with CMV enhancer and promoter
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: VP1–VP4
• Alt name: Enterovirus polyprotein
• Alt name: Coxsackievirus polyprotein
• Alt name: CVB3 polyprotein
• Species: H. sapiens (human); Coxsackievirus B3
• Insert Size (bp): 2565
• GenBank ID: M33854.1
• Promoter: Polyhedrin

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GATAATTAAAATGATAACCATC
• 3′ sequencing primer: TATAATCTTTAGGGTGGTATGTTAGAGCGA

Gene/Insert 2

• Gene/Insert name: 3CD
• Alt name: 3CD protease
• Alt name: enterovirus protease
• Alt name: CVB3 3CD protease
• Species: H. sapiens (human); Coxsackievirus B3
• Insert Size (bp): 1941
• GenBank ID: M33854.1
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: CCCTTGCTGTCCTGCCCCACCCCACCCCC
• 3′ sequencing primer: ATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGG

Resource Information

• Supplemental Documents:
  • pOET5-CVB3-VLP.gbk
• A portion of this plasmid was derived from a plasmid made by: Gene synthetically produced and cloned by GenScript.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry