pSD090A
(Plasmid
#218132)
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PurposeHybrid circuit displaying mmCRBN bait and driving expression of luxAB
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 218132 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSC101
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameCrbn
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Alt namemmCRBN
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SpeciesM. musculus (mouse)
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Entrez GeneCrbn (a.k.a. 2610203G15Rik, 2900045O07Rik, piL)
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Tag
/ Fusion Protein
- 434cI_RR69 (N terminal on insert)
Gene/Insert 2
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Gene/Insert nameluxAB
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSD090A was a gift from David Liu (Addgene plasmid # 218132 ; http://n2t.net/addgene:218132 ; RRID:Addgene_218132) -
For your References section:
Continuous evolution of compact protein degradation tags regulated by selective molecular glues. Mercer JAM, DeCarlo SJ, Roy Burman SS, Sreekanth V, Nelson AT, Hunkeler M, Chen PJ, Donovan KA, Kokkonda P, Tiwari PK, Shoba VM, Deb A, Choudhary A, Fischer ES, Liu DR. Science. 2024 Mar 15;383(6688):eadk4422. doi: 10.1126/science.adk4422. Epub 2024 Mar 15. 10.1126/science.adk4422 PubMed 38484051