pQE-nTR
(Plasmid
#21812)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQE-80L
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Backbone manufacturerQiagen
- Backbone size w/o insert (bp) 4700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepQE-nTR
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Alt nameTRAIL
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SpeciesH. sapiens (human)
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Insert Size (bp)504
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MutationHis tag and aa95-113 were removed from pQE-hTR
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Entrez GeneTNFSF10 (a.k.a. APO2L, Apo-2L, CD253, TANCR, TL2, TNLG6A, TRAIL)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQE-nTR was a gift from Wafik El-Deiry (Addgene plasmid # 21812 ; http://n2t.net/addgene:21812 ; RRID:Addgene_21812) -
For your References section:
Death induction by recombinant native TRAIL and its prevention by a caspase 9 inhibitor in primary human esophageal epithelial cells. Kim SH, Kim K, Kwagh JG, Dicker DT, Herlyn M, Rustgi AK, Chen Y, El-Deiry WS. J Biol Chem. 2004 Sep 17. 279(38):40044-52. 10.1074/jbc.M404541200 PubMed 15226295