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Addgene

pY3-LYS-IV
(Plasmid #218119)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218119 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    LYS-Leu2
  • Backbone manufacturer
    Samantha Martinusen
  • Backbone size (bp) 5626
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pY3-LYS-IV was a gift from Carl Denard (Addgene plasmid # 218119 ; http://n2t.net/addgene:218119 ; RRID:Addgene_218119)
  • For your References section:

    Modular and integrative activity reporters enhance biochemical studies in the yeast ER. Martinusen SG, Slaton EW, Nelson SE, Pulgar MA, Besu JT, Simas CF, Denard CA. Protein Eng Des Sel. 2024 Jan 29;37:gzae008. doi: 10.1093/protein/gzae008. 10.1093/protein/gzae008 PubMed 38696722