pY3
(Plasmid
#218116)
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Purpose(Empty Backbone) Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218116 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneself-made
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Backbone manufacturerSamantha Martinusen
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.07.12.548713v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pY3 was a gift from Carl Denard (Addgene plasmid # 218116 ; http://n2t.net/addgene:218116 ; RRID:Addgene_218116) -
For your References section:
Modular and integrative activity reporters enhance biochemical studies in the yeast ER. Martinusen SG, Slaton EW, Nelson SE, Pulgar MA, Besu JT, Simas CF, Denard CA. Protein Eng Des Sel. 2024 Jan 29;37:gzae008. doi: 10.1093/protein/gzae008. 10.1093/protein/gzae008 PubMed 38696722
Map uploaded by the depositor.
