Anti-DYKDDDDK [IPI-4E11/M1]
(Antibody
#218106)
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PurposeAnti-DYKDDDDK (Flag) tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds in a calcium-dependent manner to the N-terminal DYKDDDDK sequence.
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Depositing Lab
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Recommended Applications
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Recombinant Antibody | 218106-rAb | 100 µg of purified recombinant antibody | $250 * |
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Features
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Source SpeciesO. cuniculus (rabbit)
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IsotypeIgG1
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Light Chain Typekappa
Production & Usage
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Production PlasmidspTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC, pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
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Recommended Secondary AntibodyAnti-rabbit IgG
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PurificationPurification from cell culture supernatant using affinity chromatography followed by buffer exchange with centrifugal columns.
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Storage BufferPBS, 1X pH 7.4
Delivery
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Concentration0.9–1.1 mg/mL
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StorageUpon receipt, aliquot and store at 4 °C until use.
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ShipmentBlue ice
Resource Information
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Persistent Resource IdentifierRRID:AB_3095605
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Zeocin® is an InvivoGen trademark.
Trademarks:
Target Antigen
Protein | DYKDDDDK |
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Antigen Description | N-terminal DYKDDDDK |
Antigen Amino Acid Sequence |
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Species | Synthetic |
Applications (1)
Western Blot
Depositor Comments
These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Anti-DYKDDDDK [IPI-4E11/M1] - from Institute for Protein Innovation (IPI) (Addgene antibody # 218106 ; http://n2t.net/addgene:218106 ; RRID:AB_3095605) -
For your References section:
A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification. Hopp TP, Prickett KS, Price VL, Libby RT, March CJ, Cerretti DP, Urdal DL, Conlon PJ. Bio/Technology volume 6, pages 1204–1210 (1988) 10.1038/nbt1088-1204
This antibody performs best when the target is an N-terminal Flag fusion.
The heavy chain and light chain amino acid sequences are available by request after purchase. Please submit requests to [email protected]. For all other questions, please send an email to [email protected] for assistance.