Anti-EE [IPI-3D5EE_48.K]
(Antibody
#218103)
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PurposeAnti-EE tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to EYMPME sequence.
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Depositing Lab
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Recommended Applications
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Recombinant Antibody | 218103-rAb | 100 µg of purified recombinant antibody | $250 * |
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Features
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Source SpeciesO. cuniculus (rabbit)
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IsotypeIgG1
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Light Chain Typekappa
Production & Usage
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Production PlasmidspTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC, pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa
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Recommended Secondary AntibodyAnti-rabbit IgG
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PurificationPurification from cell culture supernatant using affinity chromatography followed by buffer exchange with centrifugal columns.
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Storage BufferPBS, 1X pH 7.4
Delivery
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Concentration0.9–1.1 mg/mL
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StorageUpon receipt, aliquot and store at 4 °C until use.
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ShipmentBlue ice
Resource Information
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Persistent Resource IdentifierRRID:AB_3095602
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Zeocin® is an InvivoGen trademark.
Trademarks:
Target Antigen
Protein | EE |
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Antigen Amino Acid Sequence |
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Species | Synthetic |
Alternative Names |
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Applications (1)
Western Blot
Depositor Comments
These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Anti-EE [IPI-3D5EE_48.K] - from Institute for Protein Innovation (IPI) (Addgene antibody # 218103 ; http://n2t.net/addgene:218103 ; RRID:AB_3095602) -
For your References section:
Conversion of scFv peptide-binding specificity for crystal chaperone development. Pai JC, Culver JA, Drury JE, Motani RS, Lieberman RL, Maynard JA. Protein Eng Des Sel. 2011 May;24(5):419-28. doi: 10.1093/protein/gzq120. Epub 2011 Jan 8. 10.1093/protein/gzq120 PubMed 21217145
The heavy chain and light chain amino acid sequences are available by request after purchase. Please submit requests to [email protected]. For all other questions, please send an email to [email protected] for assistance.