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Addgene

pCDNA3-hCD2
(Plasmid #21810)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21810 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4577
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1255
  • GenBank ID
    NM_001767.2 NP_001758.1
  • Entrez Gene
    CD2 (a.k.a. LFA-2, SRBC, T11)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site BstBI (not destroyed)
  • 5′ sequencing primer SV40pro-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCDNA3 Mammalian expression vector in which the NeoR selection marker has been replaced by the coding region of human CD2, a surface protein that is recognizable by various commercially-available fluorescent conjugated antibodies

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-hCD2 was a gift from David Ron (Addgene plasmid # 21810 ; http://n2t.net/addgene:21810 ; RRID:Addgene_21810)
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