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Addgene

eIF2a 3
(Plasmid #21809)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21809 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA.CD2
  • Backbone manufacturer
    Addgene plasmid 21810
  • Backbone size w/o insert (bp) 5832
  • Vector type
    Mammalian Expression
  • Selectable markers
    CD2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eIF2a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1683
  • Mutation
    S52D
  • Entrez Gene
    EIF2S1 (a.k.a. EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Bases changed to make S52D mutation, creates a Taq1 site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eIF2a 3 was a gift from David Ron (Addgene plasmid # 21809 ; http://n2t.net/addgene:21809 ; RRID:Addgene_21809)
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