eIF2a 2
(Plasmid #21808)

• Depositing Lab: David Ron
• Publication: eIF2-alpha constructs (unpublished)

Backbone

• Vector backbone: pcDNA.CD2
• Backbone manufacturer: Addgene plasmid 21810
• Backbone size w/o insert (bp): 5832
• Vector type: Mammalian Expression
• Selectable markers: CD2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eIF2a
• Species: H. sapiens (human)
• Insert Size (bp): 1683
• Mutation: S52A
• Entrez Gene: EIF2S1 (a.k.a. EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results show small insertion in the MCS, upstream of EcoRI site. ORF is not affected. This insert is one of a few discrepancies between Addgene's quality control sequence and the sequence provided by the Ron lab. These discrepancies should not affect the function of the plasmid, but they have changed some of the restriction sites in the MCS. Please proceed cautiously when doing diagnostic digests or subcloning.