pcDNA-Dest53-SHP-1 S591D
(Plasmid #21803)

• Depositing Lab: Stephen Shaw
• Publication: Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Backbone

• Vector backbone: pcDNA-Dest53
• Backbone size w/o insert (bp): 7767
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SHP-1
• Species: H. sapiens (human)
• Insert Size (bp): 1785
• Mutation: changed Serine 591 to Aspartic acid
• Entrez Gene: PTPN6 (a.k.a. HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: gataway cloning (not destroyed)
• 3′ cloning site: gataway cloning (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmids are derived from a construct originally from Dr. Taolin Yi

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry