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Addgene

pET-22b(+)_PelB-BN0034-5-FLAG-His
(Plasmid #218027)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218027 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-22b(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BN0034-5
  • Species
    Synthetic
  • Tags / Fusion Proteins
    • PelB (N terminal on backbone)
    • 1xFLAG 10xHis (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Amino Acid Sequence of Insert: QVQFVESGGGLVQPGGSLRLSCAASGFALDNYAIGWFRQAPGKEREGISCISTIDALTYYSDSAAGRFTTSRSNDRNTVYLQMNSLKPEDTGTYYCAAARGPFTLCQSDAFTYDMDYWGKGTPVTVSS.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-22b(+)_PelB-BN0034-5-FLAG-His was a gift from RESOLUTE Consortium & Giulio Superti-Furga (Addgene plasmid # 218027 ; http://n2t.net/addgene:218027 ; RRID:Addgene_218027)

  • For your References section:

    Protein binder toolbox for studies of solute carrier transporters. Gelová Z, Ingles-Prieto A, Bohstedt T, Frommelt F, Chi G, Chang YN, Garcia J, Wolf G, Azzollini L, Tremolada S, Scacioc A, Hansen JS, Serrano I, Droce A, Cuesta Bernal J, Burgess-Brown NA, Carpenter EP, Durr KL, Kristensen P, Geertsma ER, Stefanic S, Scarabottolo L, Wiedmer T, Puetter V, Sauer DB, Superti-Furga G. J Mol Biol. 2024 Jun 13:168665. doi: 10.1016/j.jmb.2024.168665. 10.1016/j.jmb.2024.168665 PubMed 38878854
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