AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3
(Plasmid
#21800)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21800 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePSMD14
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SpeciesM. musculus (mouse)
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Insert Size (bp)1645
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Entrez GenePsmd14 (a.k.a. Pad1, Poh1, rpm11, AA986732, 2610312C03Rik, 3200001M20Rik)
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Tags
/ Fusion Proteins
- TEV (C terminal on insert)
- GST (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3 was a gift from David Ron (Addgene plasmid # 21800 ; http://n2t.net/addgene:21800 ; RRID:Addgene_21800) -
For your References section:
An arsenite-inducible 19S regulatory particle-associated protein adapts proteasomes to proteotoxicity. Stanhill A, Haynes CM, Zhang Y, Min G, Steele MC, Kalinina J, Martinez E, Pickart CM, Kong XP, Ron D. Mol Cell. 2006 Sep 15. 23(6):875-85. 10.1016/j.molcel.2006.07.023 PubMed 16973439
