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pMBS-838
(Plasmid #217994)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217994 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    derived from pLEXSY_I-blecherry3 (Jena Bioscience)
  • Backbone manufacturer
    self-made
  • Backbone size (bp) 7710
  • Modifications to backbone
    endogenous BsaI restriction sites mutated via point mutation, introduction of lacZ alpha cassette for blue-white screening
  • Vector type
    Synthetic Biology
  • Promoter T7 promotor
  • Selectable markers
    lacZ alpha (E. coli), mCherry + Bleomycin (L. tarentolae)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MoClo L1 destination vector

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMBS-838 was a gift from Michael Schroda (Addgene plasmid # 217994 ; http://n2t.net/addgene:217994 ; RRID:Addgene_217994)

  • For your References section:

    A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae. Hieronimus K, Donauer T, Klein J, Hinkel B, Spanle JV, Probst A, Niemeyer J, Kibrom S, Kiefer AM, Schneider L, Husemann B, Bischoff E, Mohring S, Bayer N, Klein D, Engels A, Ziehmer BG, Stiebeta J, Moroka P, Schroda M, Deponte M. Microb Cell. 2024 Apr 30;11:128-142. doi: 10.15698/mic2024.04.821. eCollection 2024. 10.15698/mic2024.04.821 PubMed 38799406
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