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Addgene

pOT_7-lenti-EFS-SS1-BBz-2A-puro
(Plasmid #217990)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217990 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    lentiCRISPR v2
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SS1-BBz CAR
  • Species
    H. sapiens (human), Synthetic

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.27.625752 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOT_7-lenti-EFS-SS1-BBz-2A-puro was a gift from Neville Sanjana (Addgene plasmid # 217990 ; http://n2t.net/addgene:217990 ; RRID:Addgene_217990)
  • For your References section:

    Paired CRISPR screens to map gene regulation in cis and trans. Xue X, Gajic ZZ, Caragine CM, Legut M, Walker C, Kim JYS, Wang X, Yan RE, Wessels H-H, Lu C, Bapodra N, Gürsoy G, Sanjana NE. bioRxiv 2024.11.27.625752 10.1101/2024.11.27.625752