H6Gb1-CREB(85-160)
(Plasmid
#217984)
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Purposebacterial expression of CREB1 domains
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 217984 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepet21
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCREB1
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SpeciesR. norvegicus (rat)
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Insert Size (bp)480
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Entrez GeneCreb1 (a.k.a. Creb)
- Promoter T7
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Tag
/ Fusion Protein
- His6-GB1-tev (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
H6Gb1-CREB(85-160) was a gift from Peter Wright (Addgene plasmid # 217984 ; http://n2t.net/addgene:217984 ; RRID:Addgene_217984) -
For your References section:
Glutamine-rich regions of the disordered CREB transactivation domain mediate dynamic intra- and intermolecular interactions. Martinez-Yamout MA, Nasir I, Shnitkind S, Ellis JP, Berlow RB, Kroon G, Deniz AA, Dyson HJ, Wright PE. Proc Natl Acad Sci U S A. 2023 Nov 21;120(47):e2313835120. doi: 10.1073/pnas.2313835120. Epub 2023 Nov 14. 10.1073/pnas.2313835120 PubMed 37971402
