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Addgene

H6Gb1-CREB(1-280) C90A, T172C
(Plasmid #217983)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217983 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet21
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CREB1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    848
  • Mutation
    C90A, T172C
  • Entrez Gene
    Creb1 (a.k.a. Creb)
  • Promoter T7
  • Tag / Fusion Protein
    • His6-GB1-tev (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H6Gb1-CREB(1-280) C90A, T172C was a gift from Peter Wright (Addgene plasmid # 217983 ; http://n2t.net/addgene:217983 ; RRID:Addgene_217983)

  • For your References section:

    Glutamine-rich regions of the disordered CREB transactivation domain mediate dynamic intra- and intermolecular interactions. Martinez-Yamout MA, Nasir I, Shnitkind S, Ellis JP, Berlow RB, Kroon G, Deniz AA, Dyson HJ, Wright PE. Proc Natl Acad Sci U S A. 2023 Nov 21;120(47):e2313835120. doi: 10.1073/pnas.2313835120. Epub 2023 Nov 14. 10.1073/pnas.2313835120 PubMed 37971402
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