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pSAD-5PSD95-EGFP-SynPhRFP
(Plasmid #217979)

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Item Catalog # Description Quantity Price (USD)
Plasmid 217979 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSAD
  • Total vector size (bp) 18491
  • Vector type
    G-Deleted Rabies

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    5PSD95-EGFP
  • Alt name
    Postsynaptic Density Protein-EGFP
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2946
  • Entrez Gene
    Dlg4 (a.k.a. Dlgh4, PSD95, Sap90)
  • Promoter None
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    SynPhRFP
  • Alt name
    Synaptophysin-RFP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1695
  • Entrez Gene
    Syp (a.k.a. A230093K24Rik, Syn, p38)
  • Promoter None
  • Tag / Fusion Protein
    • RFP (C terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSAD-5PSD95-EGFP-SynPhRFP was a gift from Edward Callaway (Addgene plasmid # 217979 ; http://n2t.net/addgene:217979 ; RRID:Addgene_217979)

  • For your References section:

    Postsynaptic cell type and synaptic distance do not determine efficiency of monosynaptic rabies virus spread measured at synaptic resolution. Patino M, Lagos WN, Patne NS, Miyazaki PA, Bhamidipati SK, Collman F, Callaway EM. Elife. 2023 Dec 14;12:e89297. doi: 10.7554/eLife.89297. 10.7554/eLife.89297 PubMed 38096019
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