pAAV-nef-AO-66-71-TVA950
(Plasmid
#217977)
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PurposeAAV Transfer plasmid to express TVA950 in a flp-dependent manner
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 217977 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
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How this works
- Place a request for a quantity of 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
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Backbone
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Vector backbonepAAV
- Total vector size (bp) 5860
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTVA950
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Insert Size (bp)468
- Promoter nEF
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBR322ori-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-nef-AO-66-71-TVA950 was a gift from Edward Callaway (Addgene plasmid # 217977 ; http://n2t.net/addgene:217977 ; RRID:Addgene_217977) -
For your References section:
Postsynaptic cell type and synaptic distance do not determine efficiency of monosynaptic rabies virus spread measured at synaptic resolution. Patino M, Lagos WN, Patne NS, Miyazaki PA, Bhamidipati SK, Collman F, Callaway EM. Elife. 2023 Dec 14;12:e89297. doi: 10.7554/eLife.89297. 10.7554/eLife.89297 PubMed 38096019