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Addgene

pAAV-hSyn-FLEX-H2BmTagBFP2-oG
(Plasmid #217976)

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Item Catalog # Description Quantity Price (USD)
Plasmid 217976 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Total vector size (bp) 7275
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    H2BmTagBFP2
  • Alt name
    Histone2B-BFP
  • Species
    Synthetic
  • Insert Size (bp)
    1107
  • Promoter hSyn
  • Tag / Fusion Protein
    • mTAGBFP2 (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    oG
  • Alt name
    Codon-optimized Rabies G protein
  • Species
    Synthetic
  • Insert Size (bp)
    1575
  • Promoter hSyn

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-FLEX-H2BmTagBFP2-oG was a gift from Edward Callaway (Addgene plasmid # 217976 ; http://n2t.net/addgene:217976 ; RRID:Addgene_217976)

  • For your References section:

    Postsynaptic cell type and synaptic distance do not determine efficiency of monosynaptic rabies virus spread measured at synaptic resolution. Patino M, Lagos WN, Patne NS, Miyazaki PA, Bhamidipati SK, Collman F, Callaway EM. Elife. 2023 Dec 14;12:e89297. doi: 10.7554/eLife.89297. 10.7554/eLife.89297 PubMed 38096019
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