pAAV-EF1a-fDIO-stGtACR2-FusionRed
(Plasmid
#217975)
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PurposeAAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent manner
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 217975 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
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Backbone
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Vector backbonepAAV
- Total vector size (bp) 7422
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namestGtACR2-FusionRed
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Alt nameACR2
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SpeciesSynthetic; Guillardia theta
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Insert Size (bp)1839
- Promoter EF1a
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Tag
/ Fusion Protein
- FusionRed (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBR322ori-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1a-fDIO-stGtACR2-FusionRed was a gift from Edward Callaway (Addgene plasmid # 217975 ; http://n2t.net/addgene:217975 ; RRID:Addgene_217975) -
For your References section:
Distinct "driving" versus "modulatory" influences of different visual corticothalamic pathways. Kirchgessner MA, Franklin AD, Callaway EM. Curr Biol. 2021 Dec 6;31(23):5121-5137.e7. doi: 10.1016/j.cub.2021.09.025. Epub 2021 Oct 5. 10.1016/j.cub.2021.09.025 PubMed 34614389