AIRAP 7: AIRAPmyc.pBABEpuro
(Plasmid
#21797)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBABE-puro
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Backbone manufacturerWeinberg lab
- Backbone size w/o insert (bp) 5169
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAIRAP
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SpeciesM. musculus (mouse)
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Insert Size (bp)512
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GenBank IDAF224494
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Entrez GeneZfand2a (a.k.a. Airap, AA407930, AU016206)
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Tag
/ Fusion Protein
- myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBabe-5 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene sequence shows no Myc tag in this construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AIRAP 7: AIRAPmyc.pBABEpuro was a gift from David Ron (Addgene plasmid # 21797 ; http://n2t.net/addgene:21797 ; RRID:Addgene_21797) -
For your References section:
An arsenite-inducible 19S regulatory particle-associated protein adapts proteasomes to proteotoxicity. Stanhill A, Haynes CM, Zhang Y, Min G, Steele MC, Kalinina J, Martinez E, Pickart CM, Kong XP, Ron D. Mol Cell. 2006 Sep 15. 23(6):875-85. 10.1016/j.molcel.2006.07.023 PubMed 16973439