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Addgene

pCL1333
(Plasmid #217966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-MCN-6His
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RAN
  • Species
    H. sapiens (human)
  • Entrez Gene
    RAN (a.k.a. ARA24, Gsp1, TC4)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCL1333 was a gift from Andrew Bowman (Addgene plasmid # 217966 ; http://n2t.net/addgene:217966 ; RRID:Addgene_217966)

  • For your References section:

    A specific role for importin-5 and NASP in the import and nuclear hand-off of monomeric H3. Pardal AJ, Bowman AJ. Elife. 2022 Sep 6;11:e81755. doi: 10.7554/eLife.81755. 10.7554/eLife.81755 PubMed 36066346
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