Skip to main content
Addgene

pCL1161
(Plasmid #217964)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217964 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    ThermoFisher
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone H3.1
  • Species
    H. sapiens (human)
  • Entrez Gene
    H3C1 (a.k.a. H3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP, APEX (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCL1161 was a gift from Andrew Bowman (Addgene plasmid # 217964 ; http://n2t.net/addgene:217964 ; RRID:Addgene_217964)

  • For your References section:

    A specific role for importin-5 and NASP in the import and nuclear hand-off of monomeric H3. Pardal AJ, Bowman AJ. Elife. 2022 Sep 6;11:e81755. doi: 10.7554/eLife.81755. 10.7554/eLife.81755 PubMed 36066346
Related items: