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Addgene

pCL1032
(Plasmid #217962)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESpuro2
  • Backbone manufacturer
    Clonetech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NASP
  • Alt name
    Nuclear autoantigenic sperm protein
  • Species
    H. sapiens (human)
  • Entrez Gene
    NASP (a.k.a. FLB7527, HMDRA1, PRO1999)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pEGFP-C2 FP
  • 3′ sequencing primer SV40-RP
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCL1032 was a gift from Andrew Bowman (Addgene plasmid # 217962 ; http://n2t.net/addgene:217962 ; RRID:Addgene_217962)

  • For your References section:

    Evidence for the nuclear import of histones H3.1 and H4 as monomers. Apta-Smith MJ, Hernandez-Fernaud JR, Bowman AJ. EMBO J. 2018 Oct 1;37(19):e98714. doi: 10.15252/embj.201798714. Epub 2018 Sep 3. 10.15252/embj.201798714 PubMed 30177573
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